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The effect of wheat products on the colonization of the microbiota in the intestines of zebrafish (903.2)
Author(s) -
Savarese Mary,
Tousignant K. Davis,
Uno Jennifer
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.903.2
Subject(s) - dysbiosis , biology , zebrafish , gluten , microbiology and biotechnology , gut flora , antibiotics , bacteria , lactobacillus , immunology , food science , gene , genetics
Celiac disease is sensitivity to the wheat protein, gluten, characterized by intestinal inflammation and villous atrophy of the small intestine in genetically susceptible individuals. Inducing factors of celiac disease include ingestion of gluten, antibiotic exposure, infections, and a dysbiosis of the microbiota. Zebrafish have emerged as a viable model to study gastrointestinal diseases; however, they have yet to be used to directly study celiac disease. The aim of this work was to assess the potential use of zebrafish as a model for studying celiac disease. Fish were placed on a wheat‐based or brine fish regime. Antibiotics were used to knock‐down the microbiota in order to examine the reestablishment of the gut flora. All fish intestines were pooled into groups of 3, bacterial DNA was isolated and primers were used to amplify the 16S gene of specific bacterial groups. Bacterial levels were calculated as a percentage relative to total 16 S bacteria. Results show no significant difference in total bacterial expression however there was a significant decrease in Bifidobacterium (n=3, p< 0.05) in the wheat fed relative to the brine shrimp fed fish. These results confirm current studies indicating decreased expression of Bifidobacterium in bacterial DNA isolated from celiac patients. These data indicate the potential use of zebrafish to further the understanding of gut inflammation associated with wheat allergies. Grant Funding Source : Supported by Elon University