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Conserved polarization of Na,K‐ATPase in vertebrate skin epithelia (895.1)
Author(s) -
Modyanov NIikolai,
Korneenko Tatyana,
Shakhparonov Mikhail,
Pestov Nikolay
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.895.1
Subject(s) - stratum granulosum , epidermis (zoology) , gene isoform , atpase , epithelial polarity , xenopus , biology , microbiology and biotechnology , immunohistochemistry , stratum spinosum , epithelium , protein subunit , chemistry , enzyme , biochemistry , anatomy , gene , stratum corneum , genetics , immunology
Development of epidermis creates stratified epithelium with different sets of ion‐transporting enzymes in its layers. We have characterized expression of Na,K‐ and H,K‐ATPases (X=Na or H) in rat skin including all α and β subunits and six FXYD isoforms. Na,K‐ATPase is predominantly represented by α1 and β3 isoforms. Among H,K‐ATPases, only the α‐subunit of non‐gastric H,K‐ATPase (αng) was detected. Expression of ATP1A1, ATP1B4 and FXYD6 genes are down‐regulated in adult rat skin as compared to the newborn one, whereas the level of αng and FXYD4/CHIF are higher in adult skin. Immunohistochemistry with the use of monoclonal antibodies indicates that αng and α1 are differentially expressed during keratinization: α1 is gradually decreased whereas αng is enriched in the outer live cell layer, stratum granulosum. Na,K‐ATPase α1 is abundant in cells of the innermost layer, stratum basale, and, unexpectedly, is completely undetectable in their basal membranes. Epidermis of a frog species, Xenopus laevis, also show lateroapical location of Na,K‐ATPase, as well as in the chicken epidermis. These findings indicate that Na,K‐ATPase in epidermis is not basolateral as it is in most epithelial cells. On the other hand, Na,K‐ATPase is basolateral in epithelium of the cutaneous secretory glands. These features appear to be conserved in tetrapod vertebrates.Supported by RFBR grant 13‐04‐01413. Grant Funding Source : RFBR grant 13‐04‐01413