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Telemetry‐based recording of renal cortical oxygenation in response to angiotensin II and phenylephrine in the conscious rat (890.3)
Author(s) -
Koeners Maarten,
Emans Tonja,
Ow Connie,
Evans Roger,
Joles Jaap,
Malpas Simon
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.890.3
Subject(s) - renal cortex , medulla , medicine , hyperoxia , phenylephrine , angiotensin ii , renal medulla , cortex (anatomy) , hypoxia (environmental) , cardiology , kidney , blood pressure , chemistry , oxygen , neuroscience , biology , lung , organic chemistry
Previously we have developed unique technology which allows wireless measurement of the partial pressure of oxygen (PO2) in the renal medulla in unrestrained rats. The aim of our current study was to determine whether this modality can reliably measure tissue PO2 in the cortex and its responses to angiotensin‐II (AngII) and phenylephrine (PE). Cortical PO2 values were stable over time (3 weeks) and comparable between animals (Wistar, n = 8). Responses to systemic hypoxia and hyperoxia were reproducible over this time period, and were similar to those previously observed in the renal medulla. Injection of AngII in conscious rats via an implanted catheter in the femoral vein decreased cortical PO2 dose‐dependently. At a dose of 500 ng/kg a maximum decrease of 45 ± 10% was observed. In contrast equi‐pressor doses of PE had no or little effect on cortical PO2. A maximum decrease in PO2 of 8 ± 5% was observed at 1500 ng/kg. There was little evidence of fibrosis or scarring after three weeks of electrode implantation. This technology provides the opportunity to examine the temporal relationships between renal cortical tissue hypoxia and the progression of renal disease. Grant Funding Source : EU, FP7, Marie Curie Actions, International Outgoing Fellowship