Premium
Exercise training combined with insulin attenuates cardiac mitochondrial dysfunctions in diabetic rats (884.9)
Author(s) -
Cunha Daise,
Silva Márcia,
Natali Antonio,
Silva Edson,
Teodoro Bruno,
Drummond Lucas,
Drummond Filipe,
Moura Anselmo,
Belfort Felipe,
Oliveira Alessandro,
PrímolaGomes Thales,
Maldonado Izabel,
Alberici Luciane
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.884.9
Subject(s) - mitochondrial permeability transition pore , insulin , mptp , diabetes mellitus , mitochondrion , endocrinology , medicine , streptozotocin , cardiac function curve , aerobic exercise , type 2 diabetes , chemistry , heart failure , biochemistry , apoptosis , programmed cell death , dopamine , dopaminergic
Exercise training combined with insulin attenuates cardiac mitochondrial dysfunctions in diabetic rats Daise N.Q. Da Cunha 1 , Márcia F. da Silva 3 , Antonio J. Natali 2 , Edson da Silva 3 , Gilton G. Jesus 2 , Bruno G. Teodoro 4 , , Lucas R. Drummond 2 , Filipe R. Drummond 2 , Anselmo G. Moura 2 , Felipe G. Belfort 2 , Alessandro Oliveira 5 , Thales N. Prímola‐Gomes 2 , Izabel R.S.C. Maldonado 3 , Luciane C. Alberici 4 . Physical Education Department 1 , Veterinary Medicine Department 2 , General Biology Department 3 , Physics and Chemistry Department 4 , Nutrition and Health Department 5 . 1,2,3, and 5 UFV, Viçosa, and 4 USP, Ribeirão Preto, Brazil Type 1 diabetes mellitus (T1DM) leads to cardiac mitochondrial dysfunction. We tested whether aerobic exercise training combined with insulin therapy attenuate the deleterious effects of T1DM on the cardiac mitochondrial function. T1DM was induced by Streptozotocin (IP, 60 mg/kg) in male Wistar rats. They were submitted to swimming training (90 min/day, 5 days/week, 5% body mass) and received daily insulin, 4 IU, for 8 weeks. Mitochondrial function was assessed in isolated cardiac mitochondria. Diabetes increased mitochondrial Ca 2+ uptake and the susceptibility of the mitochondrial permeability transition pore (MPTP) opening, augmented resting respiration rates and reduced mitochondrial H 2 O 2 release. Exercise training and insulin, singly, reduced the mitochondrial Ca 2+ uptake; while the combination of both restored the susceptibility of MPTP opening, the rates of O 2 consumption and H 2 O 2 release in diabetic rats. In conclusion, aerobic swimming training associated with insulin therapy attenuated the disruptions of mitochondrial Ca 2+ uptake, respiration rates, and H 2 O 2 release in isolated cardiac mitochondria of rats with T1DM. We used FASEB Statement of Principles for the use of animals in research. Grant Funding Source : FAPEMIG