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Effect of 6‐weeks voluntary wheel running on the sex‐hormone and cytochrome P450 aromatase of high‐fat feeding impuberism male SD rats (884.12)
Author(s) -
Yan Yi,
Xie Minhao,
Sun Jinquan,
Zhao Yan
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.884.12
Subject(s) - medicine , endocrinology , testosterone (patch) , hormone , gonad , estrogen , turnover , sex hormone binding globulin , biology , androgen , management , economics
To compare the levels of sex hormone and cytochrome P450 aromatase in gonad and extragonadal tissues of high‐fat feeding and normal diet SD rats in response to 6‐weeks voluntary wheel running. METHOD:3‐weeks SD rats were randomly assigned to normal diet sedentary(C, n=10), normal diet exercise(E, n=10), high‐fat diet sedentary(D, n=10), high‐fat diet exercise(ErD, n=10) groups. After 2‐weeks adaption, rats of E and ErD group were individually placed into the wheel‐cage to do voluntary wheel running,1 hour each half day, 5days per week for 6 weeks. Food intake and running distance were monitored daily and body weigh weekly. Serum lipids, testosterone and estradiol were determined. The protein expression of P450arom was analyzed by immunohistochemical method. RESULTS:There were significant negative effects of high‐fat diet on serum lipids, testosterone and estradiol (P0.05). High‐fat diet significantly increased the expression of P450arom in testis, quadriceps femoris (P<0.05), liver and kidney (P<0.01), but the P450arom in cardiac muscle was significantly low (P<0.05); 6‐weeks voluntary wheel running could improve these changes. CONCLUTION:Long term high fat diets have a negative impact on metabolism of sex hormone. 6 weeks voluntary wheel running can improve the sex hormone metabolism disorders induced by high fat diets, through decreasing the expression of P450arom in gonad and extragonadal tissues. Grant Funding Source : National Natural Science Foundation of China