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Cardiovascular and skeletal muscle sympathetic nerve activity correlates in the embryonic chicken ( Gallus domesticus ) (879.6)
Author(s) -
Onyemaechi Clinton,
Crossley Dane,
Hedrick Michael
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.879.6
Subject(s) - blood pressure , baroreflex , heart rate , sodium nitroprusside , medicine , vasodilation , sympathetic nervous system , vasoconstriction , phenylephrine , endocrinology , cardiology , biology , anesthesia , anatomy , nitric oxide
The embryonic chicken ( Galls domesticus ) is a classic model for quantifying changes in cardiovascular form and function. Alterations in blood pressure, heart rate and blood flow have been quantified at multiple points of the 21 day incubation period. In addition, several regulatory systems have been assessed using pharmacological manipulation of receptors. However, the integration of cardiovascular function, and the capacity for central nervous system regulation of the cardiovascular system, has not been extensively investigated for non‐mammalian vertebrates. We investigated the correlation between muscular sympathetic nervous activity (mSNA) and fluctuations in arterial pressure and heart rate of the embryonic chicken. On day 18 of incubation, embryos were surgically instrumented with bipolar silver electrodes placed around the peroneal nerve to measure mSNA. On the following day a pressure catheter was inserted into a tertiary chorioallantoic artery to monitor arterial pressure and heart rate. The embryonic cardiovascular responses to pharmacologically‐induced vasodilation and vasoconstriction were investigated with sodium nitroprusside (SNP) and phenylephrine (Phe), respectively. In recovered embryos, acute spontaneous reductions in arterial pressure were associated with an increase in mSNA activity. In contrast, not all changes in arterial pressure induced by SNP or Phe were inversely related to mSNA. The lack of a consistent correlation between mSNA and arterial pressure suggests that the sympathetic regulatory capacity in embryonic day 19 chickens is relatively undeveloped compared with the adult animal. Grant Funding Source : Supported by NSF CAREER award to DAC.