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Detraining minimizes the protective effects of exercise on stroke recovery in mice (877.9)
Author(s) -
Poinsatte Katherine,
Ortega Sterling,
Partin Alex,
Meeuwissen Anouk,
Kong Xiangmei,
Zhang Rong,
Stowe Ann
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.877.9
Subject(s) - medicine , stroke (engine) , neuroprotection , physical exercise , peripheral , population , immune system , cd8 , endocrinology , immunology , mechanical engineering , environmental health , engineering
We previously found that 3‐weeks of voluntary exercise downregulated peripheral cytotoxic CD8 T cells and phenotypically altered resident B cells compared to sedentary controls, but only in animals that maintained exercise (duration, frequency and intensity). Mice that decreased their level of exercise with each passing week were phenotypically closer to sedentary mice, indicating that the neuroprotective effects of exercise may depend on sustained physical conditioning. We hypothesized that the protective changes to immune mechanisms seen immediately following exercise would be diminished after detraining. We developed a detraining paradigm with 3 weeks of voluntary exercise followed by a 2‐week sedentary period, prior to a 60‐min transient middle cerebral artery occlusion in adult male Swiss Webster mice. While exercise significantly reduced infarct volumes (p<0.05), 2 weeks of detraining intensified stroke severity when compared to both exercise animals (p<0.0001) and sedentary controls (p<0.05). Furthermore, detraining resulted in a larger resident CD8 T cell population in the spleen compared to sedentary controls. In conclusion, voluntary exercise does not appear to produce either long‐term neuroprotection against stroke or sustained alterations to immune mechanisms. All the changes seen in the immune system immediately following exercise were lost, and potentially exacerbated, after 2 weeks of detraining. Therefore, termination of exercise prior to injury may worsen the outcome of stroke.