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Less intimal thickening and impaired elastic fiber formation in the chicken ductus arteriosus (867.14)
Author(s) -
Omori Eriko,
Akike Toru,
Kajimura Ichige,
Minamisawa Susumu,
Goda Nobuhito
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.867.14
Subject(s) - elastin , ductus arteriosus , van gieson's stain , lysyl oxidase , anatomy , elastic fiber , embryo , hatching , andrology , chemistry , medicine , microbiology and biotechnology , biology , endocrinology , extracellular matrix , pathology , immunohistochemistry , h&e stain , zoology
Object: We investigate the vascular remodeling of the chick ductus arteriosus in order to investigate the new mechanism of its closure.. Experiments and Results: We isolated the DAs from chicken embryos and neonates. Histological analysis with Elastica van‐Gieson staining demonstrated that the sparse and fragmented elastic fibers in the medium from embryo day 16, and impairment became more apparent with development. Intimal cushion formation was not clearly observed even after external hatching. Real‐time PCR analysis revealed that the expression levels of elastin and lysyl oxidase mRNAs were significantly lower in the DA than in the aorta. Genes related with the PGE 2 ‐EP4 signal pathway were not predominantly expressed in the chicken DA. Conclusion: Internal cushion formation was less prominent in the chicken DA than in the mammalian DA. On the other hand, elastic fiber formation was severely impaired in the chicken DA, which was unlikely due to the PGE 2 ‐EP4 signal pathway.