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Effects of S1P on endothelial cell reattachment on de‐cellularized HUV scaffold for vascular tissue engineering (867.13)
Author(s) -
Chen WeiMin,
Lee Hsinyu,
Lu JenHer,
Yao ChaoLing
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.867.13
Subject(s) - umbilical vein , extracellular matrix , tissue engineering , progenitor cell , microbiology and biotechnology , scaffold , chemistry , biology , stem cell , biomedical engineering , biochemistry , medicine , in vitro
Cardiovascular disease (CVD) is the leading cause of mortality and morbidity. Choosing a proper material for bypass grafting is critical. In this study, we use de‐cellularized human umbilical veins (HUV) as natural allograft scaffolds. Cells were de‐cellularized by using sodium dodecyl sulfate (SDS) and incubated the veins with medium containing 12% FBS to remove residual nucleic acids. After de‐cellularization, human umbilical vein endothelial cells (HUVECs) and endothelial progenitor cells (EPCs) were then seeded on HUV in the presence of 1μM sphingosine 1‐phosphate (S1P) in rotational cultural conditions. S1P enhances cells attachment and reduces extracellular matrix exposure, which may cause blood clotting. Coagulation tests also showed that S1P enhanced re‐cellularized HUV scaffolds are potentially suitable for vascular grafts.