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RNA‐Seq for comprehensive analysis of differences in gene programs between conduit and resistance arteries (866.5)
Author(s) -
Dippold Rachael,
Fisher Steven
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.866.5
Subject(s) - phenotype , biology , alternative splicing , gene , gene expression , myosin , gene isoform , microbiology and biotechnology , genetics
Smooth muscle can be phenotypically categorized as tonic (eg. conduit arteries) versus phasic, or mixed properties (eg. microcirculatory arteries). Characterization of the gene expression programs that underlie the phenotypic differences between conduit and microcirculatory arteries is important to understanding the differing pathologic responses of these vessels. We performed RNA‐Seq on rat aortas vs mesenteric arteries (MAs) to identify differences in their gene programs >900 gene transcripts are differentially expressed between the two groups. Gene ontology analysis reveals an enrichment of genes involved in processes that are indicative of the oxidative “slow” phenotype in the aorta and of the “fast” phenotype in the MAs. The expression of PP1 regulatory subunits fit these phenotypes: PP1 glycogen‐targeting subunit (GL) is more highly expressed in the AO (FC=7.6), while the MP/PP1 myosin‐targeting subunit (Mypt1) is more highly expressed in the MAs (FC=3.4). We have also investigated the splicing programs of the two vessel types, with a focus on genes involved with contractility as well as factors that regulate transcription and splicing. Together our results suggest broad differences in the gene programming of conduit and resistance arteries supportive of the tonic and phasic, or mixed, smooth muscle phenotypes.