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IL‐10 supplementation suppressed hypertension and inflammation in response to placental ischemia during pregnancy (860.17)
Author(s) -
Harmon Ashlyn,
Paige Adrienne,
Cornelius Denise,
Wallace Kedra,
Thomas Alexia,
Scott Jeremy,
Moseley Janae,
LaMarca Babbette
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.860.17
Subject(s) - preeclampsia , medicine , endocrinology , inflammation , immune system , blood pressure , gestation , pregnancy , immunology , biology , genetics
Women with preeclampsia (PE) develop hypertension, proteinuria, increased pro‐inflammatory and decreased regulatory immune responses (Tregs, IL‐10) contributing to increased local ET‐1. The RUPP rat model of PE exhibits a similar increase in MAP, immune imbalance and ET‐1 as women with PE. We hypothesized that IL‐10 supplementation in RUPP rats will restore T cell balance, decrease MAP and inflammation. IL‐10 was administered intraperitoneally via osmotic pumps to RUPP rats on day 14 of gestation following the RUPP procedure. On day 18 carotid catheters are inserted, and on day 19 MAP, pup weights, serum and tissues are collected. MAP in NP rats was 91+/‐2, RUPP was 122+/‐2 which decreased to 106+/‐2 mmHg in RUPP+IL‐10. Circulating CD4+ T cells were 14% gated cells in RUPP and 8% gated cells RUPP+IL‐10. IL‐6 was 33+/‐5 NP, 238+/‐96 RUPP, 40+/‐10 pg/mL RUPP+IL‐10; TNF‐α was 14.73+/‐3 NP, 69+/‐ 19 RUPP, 20+/‐11 pg/mL RUPP+IL‐10, and placental ET‐1 was 2.5 fold higher in RUPP but only 1.4 fold higher in RUPP+IL‐10 compared to NP rats. These data suggest an important role for IL‐10 supplementation to balance the inflammatory response and improve blood pressure and ET‐1 production associated with placental ischemia of pregnancy. Grant Funding Source : Supported by RO1HD067541 & T32HL105324