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Progesterone supplementation improves blood pressure and uterine artery resistance and hypoxia‐stimulated placental cytokines during pregnancy (860.16)
Author(s) -
Amaral Lorena,
Kipprono Luissa,
Wallace Kedra,
Frazier Krystal,
Moseley Janae,
Martin James,
LaMarca Babbette
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.860.16
Subject(s) - preeclampsia , hypoxia (environmental) , medicine , uterine artery , blood pressure , placenta , endocrinology , pregnancy , gestation , fetus , chemistry , biology , genetics , organic chemistry , oxygen
Preeclampsia (PE), new onset hypertension at 20 weeks of gestation, is characterized by increase uterine artery resistance (UARI), elevated TNF‐α, IL‐6, IL‐17, and sFlt‐1. We have demonstrated that PE women have significantly lower circulating progesterone (P) than normal pregnant (NP). Therefore, we hypothesized that progesterone (17‐OHP) supplementation could improve blood pressure and decrease UARI and inflammatory cytokines during PE. To address this question we examined the effect of intraperitoneally administered 17‐OHP (3.32mg/kg) on blood pressure (MAP) and UARI in the RUPP rat model of PE. In addition, we examined hypoxia‐stimulated cytokines secreted from placenta explants collected from NP subjects treated at UMMC via ELISA. MAP in NP rats (n=13) was 92 + 2; 123 + 2 in RUPP (n=18), and 116 + 1 mmHg in RUPP+17‐OHP (n=10), p <0.05. UARI was 0.78 + 0.03 in RUPP (n=4) and 0.63 + 0.038 in RUPP+17‐OHP (n=8), p <0.05. Hypoxic placental TNF‐α, IL‐6, IL‐17, and sFlt‐1 was 2.9 + 1.0, 53 + 27, 7.3 + 4, 1038 + 362 which was blunted with 1 uM progesterone to 1.8 + 7, 33 + 7, 5 + 1.5 and 623 + 133 pg/ml. In conclusion, progesterone supplementation improves UARI and hypertension and hypoxia‐stimulated cytokines during pregnancy. Grant Funding Source : Supported by Obstetrics and Gynecology, University of Mississippi Medical Center