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The Dahl S rat on normal salt diet is a spontaneous model of preeclampsia (857.5)
Author(s) -
Gillis Ellen,
Mooney Jennifer,
Williams Jan,
Garrett Michael,
Sasser Jennifer
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.857.5
Subject(s) - preeclampsia , proteinuria , endocrinology , medicine , tbars , pregnancy , excretion , urine , urinary system , fetus , oxidative stress , kidney , lipid peroxidation , biology , genetics
Preeclampsia (PE), increased BP and proteinuria during pregnancy, is detrimental to mother and fetus. There is currently no cure, and the development of new animal models is necessary to further research in this field. Here we test the hypothesis that the Dahl S rat, a known genetic model of hypertension and kidney disease, is also a spontaneous model of preeclampsia. Female Dahl S rats were implanted with a telemetry unit via the femoral artery. After a 10 day recovery period, baseline BP was recorded. Rats were placed in metabolic cages for 24 hr urine collection at baseline, mid and late pregnancy (days 10‐11, 17‐18), and urinary protein, 8‐isoprostane, and TBARS concentrations were measured via commercial assays as increased oxidative stress is also associated with preeclampsia. There was no difference in baseline BP (152±1.33 mmHg vs 151±4.2 mmHg) or proteinuria (61±9.6 mg/d vs 60±16.8 mg/d) in the pregnant rats vs virgin rats (n=5‐7). Pregnant rats exhibited increases in BP, proteinuria (Table), urinary 8‐isoprostane excretion (13.46±2.34 vs 3.14±0.52 ng/d*) and urinary TBARS excretion (224.43±31.00 vs 140.47±7.70 μmol/d, p=0.06) during late pregnancy, but no changes were observed in age‐matched virgin rats. In addition, the Dahl S rat has a smaller litter size compared to the Sprague Dawley (6.6±2.3 vs 14.4±1.9*) and exhibits intrauterine growth restriction as measured by pup weight on day 20 (1.86±0.10 vs 2.43±0.03 g*). These data suggest that the Dahl S rat has increased oxidative stress during pregnancy, possibly contributing to the exacerbation of hypertension and proteinuria during pregnancy in this strain.Mean Arterial Pressure (mmHg) Urinary Protein Excretion (mg/d) Baseline 152±1 61±10 Mid‐Pregnancy 161±2* 149±26* Late‐Pregnancy 160±2 159±48 *p<0.05 vs baseline