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Effects of treatment with aliskiren and l ‐arginine in baroreflex sensitivity in renovascular hypertensive rats (856.5)
Author(s) -
Mengal Vinícius,
Silva Paulo,
Tiradentes Renata,
Santuzzi Cintia,
Gouvea Sonia,
Abreu Glaucia
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.856.5
Subject(s) - phenylephrine , blood pressure , baroreflex , aliskiren , medicine , renovascular hypertension , sodium nitroprusside , endocrinology , heart rate , anesthesia , renin–angiotensin system , nitric oxide
In some pathological conditions such as hypertension, there is an impairment in the autonomic control of blood pressure resulting in changes in baroreflex sensitivity. In this context, this work aims to elucidate the benefits of the combination of aliskiren and L‐arginine in the control of blood pressure and the restoration of reduced baroreflex sensitivity in renovascular hypertension. Renovascular hypertension (2K1C) was produced in Male Wistar rats (140‐160 g). Seven days after was performed by plethysmography to measure systolic blood pressure. The rats were divided in groups: Sham (6), 2K1C (7), 2K1C aliskiren 50 mg/kg (8), 2K1C L‐arg 300 mg/kg (8) and 2K1C ALK + L‐arg (7). The treatment was performed for 21 days by gavage. The animals were anesthetized and fitted with femoral venous and arterial catheters for drug injection and arterial pressure recordings. We performed the direct recording of blood pressure and heart rate compared to variations in blood pressure induced with vasoactive drugs phenylephrine and sodium nitroprusside before and after administration of superoxide dismutase (SOD) mimetic tempol. At the end of the protocol, the expression of GPphox and SOD in aorta were analyzed by Western blotting. Statistical analysis was determined using 1‐way ANOVA and Tukey test. Values are expressed as mean ± SEM and the significance of P <0.05. Only the treatment with the combination of ALK + L‐arg was able to normalize systolic blood pressure. The 2K1C showed a reduction in baroreflex sensitivity after intravenous bolus of phenylephrine when compared to sham group as well as a reduction when administered in this modulation sodium nitroprusside. This reduction was restored in 2K1C after treatment with aliskiren and with the combination of aliskiren and L‐arginine in both intravenous administrations. The combination of ALK and L‐ARG was able to reduce the arterial pressure levels and increase in baroreflex sensitivity. These effects could be related a reduction in oxidative stress. Grant Funding Source : CNPq