Premium
Comparison of the antihypertensive response to selective afferent versus total renal nerve ablation in “early phase” hypertension in the Dahl salt‐sensitive rat (856.3)
Author(s) -
Foss Jason,
Osborn John
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.856.3
Subject(s) - efferent , denervation , endocrinology , afferent , medicine , blood pressure , kidney , ablation
Similar to humans, renal denervation (RDNX) attenuates hypertension (HT) in the Dahl salt‐sensitive (DS) rat, yet it is unclear whether this effect is due to ablation of afferent or efferent renal nerves (or both). The present study tested the hypothesis that the antihypertensive effect of RDNX in the early phase (3 weeks of high salt diet) of DS HT is due to ablation of afferent renal nerves, specifically. DS rats on a low salt diet (0.1% NaCl) were instrumented for telemetric measurement of arterial pressure (AP) and heart rate (HR), and housed in metabolic cages for measurement of Na+ and H2O balance. Following a baseline period, rats were fed a 4% NaCl diet for the remainder of the protocol. After 3 weeks of 4% salt, rats underwent SHAM surgery (n = 10), RDNX (n = 10) or selective ablation of afferent renal nerves by periaxonal capsaicin (renal‐CAP; n = 10). 4 weeks later, rats were sacrificed and kidneys were harvested to confirm efferent (renal NE) and afferent (renal CGRP) denervation. Following treatment, AP continued to increase in all groups and, on the final day, was similar in SHAM (162 ± 5 mmHg) and renal‐CAP (160 ± 4 mmHg) rats (p>0.05), but was significantly lower in RDNX (150 ± 3 mmHg) rats (p<0.05). There were no differences between groups in any other parameter. These results suggest that the antihypertensive effect of RDNX in the early phase of DS HT is due to the ablation of efferent rather than afferent renal nerves.