Premium
Endothelium‐independent vascular relaxation induced by whey protein: role of protein hydrolyzes by pepsin (851.19)
Author(s) -
Silva Gabriel,
Guarido Karla,
MellingerSilva Caroline,
SilvaSantos José Eduardo
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.851.19
Subject(s) - pepsin , chemistry , phenylephrine , whey protein , endothelium , hydrolysis , relaxation (psychology) , vasoactive , enzyme , medicine , endocrinology , biochemistry , biology , blood pressure
The effects of the whey protein (WP) on the vascular system have been investigated in both animals and humans, but both active substances and mechanisms involved in its effects remain unclear. In this study, samples of WP subjected to hydrolysis by pepsine for 1 min (PP0) 1, 2, 3, 4 or 5 h (PP1, PP2, PP3, PP4 and PP5, respectively), as well as the crude WP (PPCr), had their ability to cause vascular relaxation investigated. Addition of all fractions of WP (0.3, 1, 3, 5 and 10 mg/ml) in phenylephrine‐contracted aortic rings induced a concentration‐dependent relaxation in both endothelium‐intact and endothelium‐denuded vessels. Interestingly, the maximum relaxation induced by WP fractions increased from 21.9 ± 5.4, 23.1 ± 11, 33.9 ± 9.8% for PPCr, PP0, PP1 and PP2, to 77.1 ± 4.8, 77.7 ± 3.9 and 79.7 ± 3.4 for PP3, PP4 and PP5, respectively. Importantly, none of the fractions tested impaired the vascular responses to vasoactive agents after removed from the baths. This study demonstrates that hydrolyses by pepsin, one of the main proteolytic enzymes found in the digestive system, is crucial for the ability of the whey protein to cause in vitro vascular relaxation. Grant Funding Source : Supported by CNPq, Brazil; Embrapa, Brazil; and FAPERJ, Brazil.