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Hydrogen‐rich saline reducing cardiac toxicity induced by adriamycin in rats (850.6)
Author(s) -
Liu Songqing,
Liu Yunjia,
Yu Pan,
Sun Xuejun
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.850.6
Subject(s) - cardiotoxicity , saline , tunel assay , oxidative stress , intraperitoneal injection , apoptosis , antioxidant , medicine , necrosis , toxicity , pharmacology , chemistry , anesthesia , biochemistry
Background . Hydrogen, which could selectively reduce the hydroxyl radical, is considered to be a novel antioxidant. In this study, we examined the effectiveness of hydrogen rich saline solution on the prevention of cardiotoxicity induced by Adriamycin (ADR) in rats. Methods . 30 Male Sprague‐Dawley® rats were divided randomly into 3 groups. Physiologic saline , hydrogenrich Saline or nothing was administered through intraperitoneal (i.p.) injection after exposure to ADR (2.5 mg/kg/week for 6 weeks) respectively. The levels of IL‐1b, TNF‐αand 8‐OHdG in heart were quantified using enzymelinked immunosorbent assay (ELISA) kits specific for rat according to the manufacturers’ instructions (R&D systems). Results. The death rate of hydrogenrich Saline group is lower than others. Rats in the H2 group displayed a reduced degree of myocardial neutrophilic infiltrate, necrosis, hemorrhage, and spindle‐shaped interstitial cells as compared with rats in the Control group..The number of TUNEL‐positive cells were increased in the myocardium in the Physiologic saline group and Control group. H2 comparably decreased the frequency of TUNEL‐positive cells relative to the Control group. And the level of 8‐OHdG, IL‐1b and TNF‐a were decreased in the hydrogenrich Saline group. Conclusion. The results of this study demonstrate that hydrogen‐rich saline ameliorated cardiotoxicity induced by Adriamycin by reducing oxidative stress and inflammatory cascades in heart tissue.