Premium
Calcium cycling transient parameters in fortilin‐deficient mice (850.5)
Author(s) -
Rababa'h Abeer,
Singh Sonal,
Wijaya Cori,
Pinkaew Decha,
Fujise Ken,
McConnell Bradley
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.850.5
Subject(s) - calcium , chemistry , heterozygote advantage , basal (medicine) , medicine , endocrinology , contraction (grammar) , wild type , biochemistry , mutant , biology , allele , gene , insulin
Fortilin, a 172‐amino acid polypeptide with potent anti‐apoptotic activity, is ubiquitously expressed in normal tissues. It had been validated through calcium‐overlay assays that fortilin binds calcium. We studied a cardiac phenotype in fortilin‐deficient mice, utilizing cardiomyocytes as the model of study, to better understand the role of fortilin in association with calcium in regulating cardiac performance. Therefore, we isolated cardiomyocytes from wild‐type as well as fortilin‐heterozygous mice using Langendorff perfusion system. Then we measured the calcium cycling parameters via Ionoptix microscopy, at basal level and after isoproterenol (ISO) administration. Data analysis showed that the cardiomycoytes isolated from heterozygous mice displayed a significant reduction after ISO administration in both Ca2+ transient amplitude (WT: 51.38 ± 4.10, Heterozygous: 51.87 ± 7.16, WT ISO: 74.76 ± 4.72, Heterozygous ISO: 52.68 ± 5.629; p蠄 0.0025) and contraction amplitude (WT: 4.586 ± 0.88, Heterozygous: 5.437 ± 0.67, WT ISO: 9.728 ± 1.83, Heterozygous ISO: 7.182 ± 1.29; p= 0.012). However, there were no significant differences between both at basal level. These data suggest that there is a differential response to ISO treatment in fortilin deficient mice compared to wild‐type.