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Evaluation of safety and efficacy antifungal of an amide derivative of amphotericin B as scaling product on in vitro models (844.12)
Author(s) -
Rodriguez Lopez Anahí,
Ortega Ivan,
FernandezZertuche Mario,
Regla Ignacio,
ReyesEsparza Jorge,
RodríguezFragoso Lourdes
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.844.12
Subject(s) - amphotericin b , candida albicans , nephrotoxicity , corpus albicans , microbiology and biotechnology , chemistry , candida glabrata , apoptosis , pharmacology , in vitro , candida tropicalis , medicine , kidney , biology , biochemistry , antifungal
To evaluate the efficacy and safety of amide derivative of amphotericin B, as scaling product at in vitro experimental assays. Amphotericin B (AmB) has been considered the gold standard drug for treating mycosis, however, it produce nephrotoxicity, hepatotoxicity and hemolytic anemia. For evaluating the antifungal activity of the AmB and DA, we used six strains of Candida. Yeasts were seeded in 96‐well plates in SD broth media. Cells were treated with AmB and DA at concentrations of 1, 10, 50, 100, 250 and 500 mM and 0.1 to 1 µM in order to determine the IC50. Safety was evaluate in kidney and liver cells. These cells were treated with AmB and DA at different concentrations 1, 10, 50, 100, 250 and 500 μM and the citotoxicity was evaluated by MTT technique and also identified the type of cell death by immunofluorescence microscopy. The DA was effective on 6 yeast Candida species treated with AmB and DA. IC50 founds were: C. albicans (SC5314) AmB= 0.13 µM vs DA= 0.26 µM; C. albicans (10231) AmB= 0.13 µMvs DA= 0.26 µM; C. albicans (131334) AmB= 0.21 µMvs DA: 0.26 µM; C. parapsolosis AmB= 0.26 µMvs DA= 0.25 µM; C. glabrata (122246) AmB= 0.34 µMvs DA= 0.26 µM; C. tropicalis (41997) AmB= 0.29 vs DA= 0.26 µM. DA produced cell death by apoptosis at concentrations 50 μM in kidney cells, but in liver cells produced cell deth by necrosis and apoptosis at concentrations 10μM. The DA as a scaling product was more safe and effective as compared to AmB. Grant Funding Source : CONACYT