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Palmitoylethanolamide ameliorates development of colitis caused by injection of dinitrobenzene sulfonic acid in mice (840.3)
Author(s) -
Cuzzocrea Salvatore,
Campolo Michela,
Impellizzeri Daniela,
Paterniti Irene,
Bruschetta Giuseppe,
Cordaro Marika,
Esposito Emanuela
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.840.3
Subject(s) - palmitoylethanolamide , nitrotyrosine , colitis , chemistry , pharmacology , inflammatory bowel disease , neuroprotection , nitric oxide , nitric oxide synthase , biochemistry , inflammation , immunology , medicine , enzyme , receptor , cannabinoid receptor , organic chemistry , agonist , disease
Dinitrobenzene sulfonic acid (DNBS) induced colitis has proven to be a useful experimental model of intestinal bowel disease (IBD). N‐palmitoylethanolamide (PEA) is an endogenous fatty acid amide with important analgesic, anti‐inflammatory and neuroprotective effects. The aim of the study was to examine the effects of a new formulation of PEA (PEA ultramicronized) in mice subjected to experimental colitis. PEA was administered daily i.p, (10 mg/kg, 10% ethanol). Four days after DNBS, colon nuclear factor NF‐ kB was increased as well as TNF‐a and IL‐1b production, neutrophil infiltration and adhesion molecules expression. Immunohistochemistry for inducible nitric oxide synthase (iNOS), nitrotyrosine and poly (ADP‐ribose) polymerase (PARP) showed an intense staining in the inflamed colon. PEA significantly reduced the appearance of diarrhea and body weight loss. This was associated with a significant reduction of MPO activity, NF‐kB, pro‐inflammatory cytokines release, iNOS, nitrotyrosine and PARP and reduced up‐regulation of ICAM‐1, P‐selectin and metalloproteinases. Thus, the results of this study suggested that administration of PEA may be beneficial for treatment of intestinal inflammatory diseases.