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Anti‐infammatory effect of Cucurbita maxima sweet seed extract on foregut induced injury: role of oxidative stress (840.2)
Author(s) -
PshykTitko Irena,
Zayachkivska Oksana
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.840.2
Subject(s) - foregut , endocrinology , antagonist , muscarinic acetylcholine receptor , histamine , medicine , atropine , oxidative stress , histaminergic , pharmacology , muscarinic antagonist , chemistry , receptor , anatomy
The established therapy of foregut disorders has resulted in incomplete efficacy, in long term use it is risky by unexpected effects, as altered microbial profile which elicits inflammation, increased oesophageal sphincters, hypokinetic gastric dysfunctions, inhibition of activity of dimethylarginine dimethylaminohydrolase (DDAH), which increase the risk of vascular inflammation (Ghebremariam YT, 2013) etc. In this study, to investigate effect of Cucurbita Maxima sweet seed extract (CMSE) on foregut mucosal integrity during blocking gastric acid by histamine H‐2‐receptor antagonist ranitidine (R), antagonist of the muscarinic acetylcholine receptors, atropine (A) given separately or in combination with the non‐selective blocker NOS L‐NAME. Rats were used with without/with CMSE treatment (200 mg/kg) during 1 to 3 days A (3 mg/kg), R (100 mg/kg), L‐NAME (10 mg/kg) and their combination applying; oral, esophageal, gastric lesions by histology score index (HIS); NO by biochemical study, IL‐1ß, GRO/CINC‐1 via ELISA. In rats R, A and L‐NAME pre‐treatment a significant accelerated production of IL‐1ß (14,3%) GRO/CINC‐1 ‐ 191,4%, rise of HIS vs control. NO content showed increasing tendency with maximum rise after 3 days during combination of triple blocking cholinergic, histaminergic and NOS via L‐NAME administration. CMSE decrease foregut lesions and signs of inflammation and IL‐1ß and GRO/CINC‐1 secretion. Histamine and NO synthesis exert biphasic biological effect in foregut when in physiological dose are essential for natural functioning but in the high or low application seems to be a reason of cell and tissue damage. CMSE has ability to maintain foregut mucosal integrity normalizing redox system activity and inflammatory mediators. Grant Funding Source : supported by prof. S. Szabo