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Anti‐inflammatory effect of miR‐17‐3p in human umbilical vein endothelial cells (840.16)
Author(s) -
Zhang Yu,
Leung Susan,
Man Ricky
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.840.16
Subject(s) - umbilical vein , transfection , lipopolysaccharide , lipofectamine , inflammation , tumor necrosis factor alpha , microrna , blot , medicine , microbiology and biotechnology , human umbilical vein endothelial cell , immunology , biology , gene , in vitro , vector (molecular biology) , biochemistry , recombinant dna
Anti‐inflammatory effect of miR‐17‐3p in human umbilical vein endothelial cells Yu ZHANG, Susan WS LEUNG and Ricky YK MAN Department of Pharmacology & Pharmacy, the University of Hong Kong, Hong Kong SAR, China Aim: MicroRNAs (miRNAs) are a class of small, noncoding RNAs of ≍22 nucleotides that negatively regulate gene expression. They appear to play a role in the development/progression of many disorders, including inflammation, cardiovascular diseases and endothelial dysfunction. This study examines the role of miR‐17‐3p in vascular inflammation. Methods: Human umbilical vein endothelial cells (HUVECs) were transfected with miR‐17‐3p agomir (miR‐17‐3p mimic) or its negative control using lipofectamine 2000. They were incubated with lipopolysaccharide (LPS, 10 ng/ml) for 16 hours to induce inflammatory reactions. The level of miR‐17‐3p and the expressions of IκBα, p65 and phosphorylated p65 (p‐p65) were measured by qPCR and Western blotting. The amount of interleukin‐8 (IL‐8) and tumor necrosis factor (TNF)‐α released in the culture medium was detected with ELISA kit. Results: The levels of miR‐17‐3p, IL‐8, TNF‐α and p‐p65 were increased following LPS stimulation. While the expression of p65 was not changed, the expression of IκBα was reduced. LPS‐induced increase in IL‐8 level and decrease in IκBα expression were smaller in HUVECs transfected with miR‐17‐3p agomir than in those transfected with the negative control. Conclusion: MiR‐17‐3p is up‐regulated in LPS‐induced inflammation. It may produce an anti‐inflammatory effect by inhibiting NF‐κB pathway and reducing IL‐8 production.