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The role of dopaminergic signaling in Daphnia magna swimming (839.5)
Author(s) -
Barrozo Enrico,
Fowler David,
Ingebretson Justin,
Beckman Matthew
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.839.5
Subject(s) - daphnia magna , daphnia , dopaminergic , bromocriptine , agonist , dopamine , pharmacology , dopamine receptor d2 , dopamine receptor , dopamine agonist , biology , chemistry , receptor , medicine , endocrinology , biochemistry , toxicity , ecology , zooplankton , hormone , prolactin
Daphnia are freshwater crustaceans that have been used for decades in ecotoxicology research. In order to determine the role of dopamine in Daphnia movement we have carried out a screen of 10 dopamine receptor drugs; three drugs affected movement. To screen for drug effect on behavior in Daphnia we have developed a two‐dimensional video imaging system to measure the movement of Daphnia magna swimming in shallow circular‐well plates. At one, two, and six hours of treatment with 10µM drug, the D 1 ‐like receptor agonist (1 R ,3 S )‐1‐(Aminomethyl)‐3‐phenyl‐3,4‐dihydro‐1 H ‐isochromene‐5,6‐diol (A68930), the D 2 ‐like agonist (5'a)‐2‐Bromo‐12'‐hydroxy‐2'‐(1‐methyl舃ethyl)‐5'‐(2‐methylpropyl)ergotaman‐3',6',18‐trione mesylate (bromocriptine), and the D 4 ‐like receptor antagonist 3‐(4‐[4‐Chlorophenyl]piperazin‐1‐yl舃)‐methyl‐1 H ‐pyrrolo[2,3‐ b ]pyridine trihydrochloride (L‐745, 870) all decreased movement with different time‐courses of inhibition. For two of the drugs which exhibited significant effects on daphnid behavior at one hour of treatment, A68930 and bromocriptine, the IC 50 values were determined to be in the low micromolar range. Preliminary results from experiments with the dopamine neurotoxin 1‐Methyl‐4‐phenylpyridinium iodide (MPP+) are consistent with a previous study showing 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine (MPTP) treatment decreased Daphnia swimming behavior. Last, we have performed genome mining studies which have identified a suite of dopaminergic genes in the Daphnia magna genome. Taken together, the data provide evidence that specific dopamine signaling pathways are involved in Daphnia swimming behavior. We propose that Daphnia is a useful model to use in studies of dopaminergic signaling in healthy and neurotoxin‐induced disease states. Grant Funding Source : Supported by The Augsburg URGO, McNair, and NorthStar STEM Programs and the College Dean's Office