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Increase of splenic lymphocyte apoptosis in septic encephalopathy (835.12)
Author(s) -
Jeremias Isabela,
Victorino Vanessa,
Lima Thais,
Soriano Francisco
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.835.12
Subject(s) - immune system , apoptosis , spleen , sepsis , medicine , flow cytometry , encephalopathy , immunology , lymphocyte , ligation , biology , biochemistry
Sepsis is a systemic response to infection that can affect any organ system, including the central and peripheral nervous systems. Authors reported that changes in the nervous system are implicated in the induction or amplification of immune cell changes. We investigated the changes in brain function and late immune response in an animal model of sepsis by cecal ligation and puncture (CLP). Balb/c mice were divided into two groups: Sham operation or CLP. We evaluated brain changes by SHIRPA behavioral test, after 6h of CLP. 15 days after surgery, mice were sacrificed, the spleen was isolated and lymphocytic apoptosis was evaluated by flow cytometry. We observed that the neuropsychiatric state in SHIRPA test was significantly decreased in animals with CLP. Regarding apoptosis of lymphocytes, there was also a significant difference, in which the CLP apoptotic lymphocytes were greater than in the sham group. Correlating (r=‐0.96) the two factors we observed that the higher the neurological damage greater the number of apoptotic lymphocytes. Thus, we conclude from these preliminary results that neurological status observed 6 hours after CLP may determine delayed immune response impairment.Support: FAPESP 2012/07898‐0 Grant Funding Source : Support by: FAPESP 2012/07898‐0