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Optical versus impedance lamellar body counts on the Sysmex XT‐2000 automated hematology analyzer (834.13)
Author(s) -
Desai Diana,
Bennett Sterling
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.834.13
Subject(s) - hematology analyzer , amniotic fluid , lamellar granule , medicine , hematology , spectrum analyzer , respiratory distress , nuclear medicine , biomedical engineering , lung , anesthesia , fetus , pregnancy , physics , biology , optics , genetics
Respiratory distress syndrome, the ninth leading cause of newborn mortality in the US, is caused by fetal lung immaturity due to pulmonary surfactant deficiency. Lamellar body counts (LBC) are often employed for fetal lung maturity assessment. The Sysmex XT‐2000 hematology analyzer measures LBC in amniotic fluid using either impedance or optical platelet counts. The objective of this study was to compare impedance versus optical LBCs. Methods: LBC tests were performed on 18 residual frozen amniotic fluid specimens on a Sysmex XT‐2000 analyzer in an Intermountain Healthcare laboratory, following manufacturer’s instructions for impedance and optical platelet counts. Results: Results were highly correlated (r 2 =0.99). Mean impedance and optical LBC values were 60.6 and 62.1 x10 3 /uL, respectively ( p =0.0079). The mean difference between paired values was ‐2.7% (range ‐7.8% to +3.1%). Conclusions: Impedance and optical LBCs are highly comparable using the Sysmex XT‐2000 analyzer.