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A possible cell surface mechanism for magnesium sulfate's antithrombocytic activity (833.1)
Author(s) -
Zem Gregory,
Arvizu S.,
TerPapyan H.,
Sadeghi H.,
Hovsepian G.,
Hambarsoomian A.,
Evans A.,
Chavez C.,
Tivriktsyan A.,
Tortorice C..,
Ohanessian K.,
Orujyan A.,
Irikyan C..,
Molina D.,
Behshadpour A.,
Markosian S.,
Boyadjian Z.,
Salas N.,
Basmajian M.,
Aminian P.,
Patel K.,
Haritounian A.,
Kurginyan A.,
Sheikhpoor B.,
Sarkissian L.,
Tosunyan O.,
Simidyan A.,
Mikaelyan H.,
Bardo M.,
Simonyan K.,
YahyaKashani D.,
Labson K.,
Asatryan M.,
Gyurgchyan T.,
Oppenheimer S.
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.833.1
Subject(s) - mannitol , chemistry , reagent , intracellular , cell , magnesium , biophysics , biochemistry , biology , organic chemistry
MgS0 4 suppresses platelet aggregation (Brit J Hematol 119: 1033, 2002; Circulation 105: 1970, 2002; Magnes Res 18: 7, 2005; J Matern Fetal Neonat Med: 478, 2012). Intracellular metabolic mechanisms have been proposed to cause this suppression. Here we show, using a simple, inexpensive quantitative assay (FASEB J 27: abs 341, 2013) that MgSO 4 disaggregates fixed cells in a yeast ( Saccharomyces cerevisiae ) model. In 1715 separate experiments by 35 independent investigators, MgSO 4 (3mg/slide droplet) increased percent of fixed single cells by 60 min by an average of 17% to as much as 33% (with p values often less than 0.05 compared to controls without reagent), while alpha methyl glucose, D‐glucose and D‐mannitol decreased percent single cells by an average of 15%. It remains to be determined if MgSO 4 can unclump fixed platelets, implicating a cell surface mechanism in its antithrombocytic activity. MgSO 4 may be a useful anticlumping agent in many applications (Science 341: 186, 2013). Anti‐infection, anti‐biofilm, and anti‐cancer reagents could be identified using this simple, inexpensive, quantitative assay system (Supported by university incentives and grants from NIH RISE, SCORE, Sidney Stern Memorial Trust, Joseph Drown Fdn and NSF Presidential Award 0731633).