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In vivo and in vitro studies on the antioxidant activity of hesperetin and its glycosylated derivatives (830.8)
Author(s) -
Carvalho Patricia,
Franco Elida Paula,
Souza Verônica,
Araújo Maria Elisa,
Franco Yollanda,
Messias Márcia,
Priviero Fernanda
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.830.8
Subject(s) - hesperetin , hesperidin , chemistry , xanthine oxidase , antioxidant , tbars , dpph , triglyceride , glutathione peroxidase , biochemistry , flavonoid , pharmacology , lipid peroxidation , cholesterol , superoxide dismutase , enzyme , biology , medicine , alternative medicine , pathology
The effect of of hesperetin (HT), hesperidin (HG) and G‐hesperidin (GHD) on oxidative‐antioxidative system and lipid profile in rats was evaluated and correlated to their in vitro antioxidant activity. Twenty‐four macho wistar (n=6) fed with standard rat chow diet were supplemented with each flavonoid (1mmol/Kg) and saline solution (control group). TBARS, GPx, SOD and CAT levels were measured in hepatic tissue. The levels of triglyceride, total cholesterol and lipoprotein fraction cholesterol levels were measured in plasma. In vitro antioxidant was evaluated by DPPH radical‐scavenging and the kinetic parameters of xanthine oxidase inhibition were determined by Lineweaver‐Burk plots. GPx and SOD levels were significantly higher in HT and HD groups than those in control group. Both groups treated showed atherogenic index levels significantly lower compared to the control group. Hesperetin showed a greater in vitro antioxidant activity and was stronger xanthine oxidase inhibitor with IC 50 value of 53µM and K i value of 17.3µm. Although the aglycone form hesperetin showed higher scavenging activity against DPPH and the stronger xanthine oxidase inhibitor, the three compounds showed a positive change occurred in oxidative‐antioxidative balance in rats. Grant Funding Source : FAPESP 2012/20393‐4