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Hair growth promoting effects of Geranium sibiricum L extract on human dermal papilla cell under glucocorticoid‐induced stress condition (830.18)
Author(s) -
Lee BogHieu,
Yu Miri,
Choi JooYeon,
Jeong Gi Hee
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.830.18
Subject(s) - grape seed extract , cell growth , chemistry , cytokine , dexamethasone , glucocorticoid , apoptosis , medicine , endocrinology , biology , biochemistry , pathology , alternative medicine
The study investigated whether Geranium Sibiricum L extract(GSE) with high antioxidant activity has hair growth promoting effect in artificial stress condition or not. MTT assay and scratch migration assay were used for to determine the GSE effect on human dermal papilla cell(hDPC). To analyze hair growth promoting effect of GSE, we implemented Ki67 immunochemistry staining and cytokine(TGF‐β1 and HGF) analysis. Stress condition was induced using synthetic glucocorticoid (dexamethasone, DEX). GSE showed highest proliferation effect in 19.5 ppm(ug/ml) and migration effect of GSE was actively accelerated in 19.5 ppm as well. DEX treatment group appeared to be very low Ki 67 positive cells but GSE and DEX co‐treatment group exhibited Ki 67 positive cells more than normal condition(NC) group. Relative TGF‐β1 expression was the lowest in GSE group, while DEX group showed the highest TGF‐β1 expression. GSE and DEX co‐treatment group showed 25.8% lower TGF‐β1 expression than DEX treatment group. Relative HGF expression was the highest in GSE group. In DEX group, HGF expression was very low, but GSE and DEX co‐treatment group showed similar HGF expression to NC group. GSE and DEX co‐treatment group expressed 12 times more HGF expression level than DEX group. These results suggest that GSE promotes the proliferation and migration of hDPC possibly by suppressing TGF‐β1(hair growth inhibition cytokine) expression level, and elevating HGF(hair growth promoting cytokine) expression in stressful condition. Grant Funding Source : supported by Basic Science Research Program through the Natiional Research Foundation of Korea(NRF)