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Cinnamon polyphenols increase oxygen‐glucose deprivation of decreased PGE2 production by upregulation of SIRT1 and alleviation of the anti‐inflammatory effects (830.11)
Author(s) -
Qin Bolin,
Anderson Richard
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.830.11
Subject(s) - polyphenol , downregulation and upregulation , oxidative stress , proinflammatory cytokine , reactive oxygen species , chemistry , prostaglandin e2 , pharmacology , apoptosis , inflammation , biochemistry , endocrinology , medicine , antioxidant , gene
Prostaglandin E2 (PGE2) is an important mediator of many biological functions, including vasodilation, both anti‐ and proinflammatory actions, and modulation of sleep/wake cycles. It is well known that cinnamon polyphenols have anti‐oxidant properties and that oxidative stress damages endothelial cells. We investigated the mechanisms underlying protective effects of an aqueous extract of cinnamon, high in polyphenols, in 3 hr oxygen‐glucose deprivation (OGD) treated mouse brain microvascular endothelial (bEnd.3) cells. Immunoprecipitation/Western blotting and immunofluorescent analyses showed that OGD/reperfusion down regulated PGE2 secretion into the media and cellular PGE2 expression. Cinnamon polyphenols reversed these damages and also induced sirtuin (SIRT)1 expression. Further studies showed that the effects were reversed by a SIRT1 inhibitor IV . Cinnamon polyphenols also inhibited the expression of the anti‐apoptotic protein, Bcl‐xl, and induced the expression of the pro ‐ apoptotic protein, Bax, in OGD treated cells. The OGD‐induced inflammatory factors, TNF‐α and phospho‐Nf‐kB p65, were suppressed by cinnamon polyphenols. Taken together, these results suggest protective roles of cinnamon on PGE2 production and that cinnamon polyphenols may be neuroprotectants for ischemia‐related injury. Grant Funding Source : This work was supported in part by a USDA Cooperative Research and Development Agreement (CRADA No.5