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Dose response of aged garlic extract in streptozotocin ‐induced diabetic rats (829.9)
Author(s) -
Thomson Martha,
AlQattan Khaled,
J.S. Divya,
Ali Muslim
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.829.9
Subject(s) - streptozotocin , medicine , endocrinology , diabetes mellitus , glycated hemoglobin , lipid peroxidation , hemoglobin , oxidative stress , blood sugar , insulin , type 2 diabetes
The anti‐diabetic and antioxidant effects of 3 doses of aged garlic extract (AGE) were studied in diabetic rats. Diabetes was induced by IP injection of streptozotocin (STZ) and a fasting blood sugar >300 mg/dl indicated diabetes. AGE (Wakunaga‐Kyolic, USA) was administered at 3 different doses intraperitoneally (IP) – 600, 300 and 100 mg/kg ‐ daily for 8 weeks. Diabetic rats were divided into 5 groups; control, boiled AGE‐treated and the 3 AGE‐treated groups. A normal control group was also included. Effects in AGE‐treated diabetic rats were dose dependent. STZ‐induced diabetic rats lost significant weight during the experiment with AGE‐treated rats (600 and 300 mg/kg doses) maintaining their initial weights. Blood glucose as well as serum cholesterol and triglycerides were significantly elevated in diabetic rats, and lowered by AGE treatment. Serum insulin level was decreased over 10‐fold in diabetic rats and increased by AGE treatment; while erythrocyte glycated hemoglobin (GHb) increased over 3‐fold in diabetic rats with a marked decrease in AGE‐treated diabetic rats. Total antioxidant levels and catalase activity decreased (45‐70%) in serum, kidneys and livers of diabetic rats with significant recovery in AGE‐treated animals. In contrast, lipid peroxidation (MDA levels) increased in kidneys and livers of diabetic rats and was lowered in AGE‐treated diabetic rats. In general, administration of boiled AGE had few beneficial effects compared to AGE. Thus, AGE exhibits a dose‐dependent effect in ameliorating oxidative stress and other indicators of diabetes in STZ‐induced diabetic rats. The study was supported by KU grant #SL 02/13. Grant Funding Source : Supported by Kuwait University SL 02/13