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Mutagenic and apoptotic effects of white mold ( Sclerotinia sclerotiorum ) in vivo and in vitro (824.6)
Author(s) -
Roque Aline,
Kim Hyemee,
Azevedo Luciana,
Barrocas Ellen,
Machado Jose,
Trevisan Jerusa,
Santos Marcelo Henrique,
MertensTalcott Susanne
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.824.6
Subject(s) - sclerotinia sclerotiorum , comet assay , in vivo , micronucleus test , dna damage , in vitro , apoptosis , microbiology and biotechnology , genotoxicity , chemistry , biology , botany , toxicity , biochemistry , dna , genetics , organic chemistry
Various common foods, including beans, soybeans, peas, and potatoes, are often contaminated by soil fungus called white mold, Sclerotinia sclerotiorum . To data, little information is available regarding the toxicological effects. This work aimed to determine the apoptotic and mutagenic potential of aqueous extract of Sclerotinia sclerotiorum (AESS) in vivo and in vitro . Chemical analysis showed that the extract was rich in oxygenated monoterpenes, such as camphor. Swiss mice were fed 10, 100 and 1000mg/kg bodyweight of AESS for 15 days. The comet assay was performed in peripheral blood cells and the number of micronucleated polychromatic erythrocytes (MNPCEs) was determined in bone marrow cells. In addition, HT‐29 and CCD‐18 cell lines were treated with 0.005, 0.01 and 0.02ppm of AESS for performing cell viability assay and RT‐PCR. In results, the extract had mutagenic effects in the micronucleus test, by increasing the number of MNPCEs up to 439%. However, the extract did not show any DNA‐damage in the comet assay. In vitro, the extract demonstrated pro‐apoptotic effects through modulation of gene expressions associated with p53‐mediated apoptosis pathway in both cancer and non‐cancer colon cells within a low concentration range. In conclusion, the AESS did not cause DNA damage in the comet assay, but potential mutagenic and cytotoxic activities within a low concentration range should be further investigated.