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Anti‐carcinogenic effect of glyceollins in DMBA‐induced mammary tumor model (824.5)
Author(s) -
Kim Bo Ram,
Seo Ji Yeon,
Kang Hye Ryung,
Kim Seong Soon,
Zakharova Alexandra,
Kim Mi Hye,
Woo Ji Eun,
Han Mi Kyung,
Kim JongSang
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.824.5
Subject(s) - dmba , carcinogen , medicine , chemistry , endocrinology , cancer , cancer research , carcinogenesis , biology , biochemistry
Glyceollins are synthesized from daidzen in soybean elicited with Aspergillus sojae. Several studies reported that glyceollins had anti‐estrogenic, anti‐inflammatory, and anticancer activities in both breast cancer cell and animal models. This study was aimed at investigating whether glyceollins exert anticancer activity through regulation of phase 2 detoxifying enzymes. That is, I was interested in investigating if glyceollins can inhibit DMBA‐induced breast cancer development by enhancing xenobiotic metabolizing enzymes such as glutation S ‐transferase, glucuronyl transferase, and sulfotransferase. The expression of phase 2 enzymes in organs collected from mice was assessed by Western blot, immunohistochemistry(IHC). Also we observed the tumor formation in mammary grand mice by whole mount analysis. The number of fat pad TEBs in the mammary tissue was significantly increased by DMBA treated and reduced by co treatment with glyceollins. Also glyceollins were found to induce same antioxidant/detoxifying enzymes in the liver. Therefore, we suspected that DMBA had little effect on mammary tissue as it was rapidly metabolized by detoxifying enzymes induced by glyceollins. In conclusion, glyceollins apprear to confer protection to mammary tissue from chemical carcinogen. Grant Funding Source : Supported by NRF‐2010‐0027204 and 2011‐0009782 and IPET (High Value‐added Food TechnologyDevelopment Program, 2012, 112066‐3), S. Korea