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Serum metabolic profile of the 7,12‐dimethylbenz(a)anthracene (DMBA)‐induced mammary tumor in obese Zucker rat model (824.1)
Author(s) -
Hakkak Reza,
Korourian Soheila,
Pavliv Oleksanda,
Melnyk Stepan
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.824.1
Subject(s) - dmba , glutathione , methionine , medicine , endocrinology , oxidative stress , chemistry , cystine , mammary tumor , methylation , cysteine , obesity , 7,12 dimethylbenz[a]anthracene , amino acid , cancer , biochemistry , breast cancer , carcinogenesis , gene , enzyme
Obesity is epidemic and can effect the metabolic profile in animals and humans. Obesity is linked to breast cancer development. We reported that obesity significantly lower serum level of S‐adenosylmethionine (SAM) and lower methylation ratio compare to lean rats. We investigated the serum profile of tumor vs. non‐tumor rats for methylation cycle and oxidative stress metabolites using DMBA‐induced obese zucker rat. Twenty (20) six‐week‐old obese female Zucker rats were gavaged at age 50 days with 65 mg DMBA/kg body weight and were sacrificed 155 days later. Serum concentration of tumor and non‐tumor bearing rats were measured for oxidative stress and methylation cycle metabolites: SAM, S‐adenosylhomocysteine (SAH), Methionine, reduced glutathione (GSH), oxidized glutathione (GSSG), Cysteine and Cystine by using HPLC and LC‐MS. We found a significant (P=0.004) decrease of GSH/GSSG ratio and significant (P=0.022) increase of Cystine/Cysteine ratio in tumor bearing rats. Despite a slight increase in concentration of free GSH and free Cysteine serum concentration also we found a significant higher (P=0.014) concentration of Methionine and SAM (P=0.038) in non‐tumor rats. Our results suggest that tumor bearing rats has higher level of oxidative stress which can affect methylation capacities of tumor bearing rats by decreasing concentration of amino acid Methionine and SAM concentration. Grant Funding Source : Arkansas Bioscience Inst