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Effects of dairy ingredients on cytokine responses to LPS challenge in mice (822.5)
Author(s) -
Price Tara,
Baskaran Sangeetha,
Walzem Rosemary
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.822.5
Subject(s) - cytokine , lipopolysaccharide , immune system , lysozyme , chemistry , inflammation , tumor necrosis factor alpha , immunology , biology , endocrinology , medicine , food science , biochemistry
Gut permeability to bacterial cell wall components, e.g.LPS, are thought to play a causative role in chronic systemic inflammation leading to disease. The effects of individual protein sources, namely isolated soy protein (ISP), dried whole milk powder (DWMP), milk fat globule membrane (MFGM) or milk protein concentrate (MPC) on cytokine responses to LPS (Salmonella) challenge was studied using whole blood from C57BL/6J mice (n=10/group) following 13 wk of test diet feeding. Isocaloric and isonitrogenous diets provided 20 EN% protein, 50 EN% carbohydrate and 30 EN% fat. Mice grew and consumed feed similarly. Multiplex assay for basal inflammatory cytokines showed lowest values for DWMP in all cases and significant for IL‐1β and TNF‐α (p<0.05). No differences in basal IL‐10 were observed. In response to LPS challenge IL‐12(p70), IFN‐γ, TNF‐α and MIP‐2 were unchanged. Fold increases in IL‐1β and IL‐6 were lowest for MPC while ISP and DWMP showed the greatest increase in IL‐1β and IL‐6, respectively (p<0.05). IL‐10 was similar in MFGM and MPC but MFGM was significantly higher than ISP and DWMP after LPS challenge. Dairy protein components appear to differentially influence cytokine response to LPS. Intact dairy protein (DWMP), appears least inflammatory in a normal gut, while specific milk protein fractions (MPC and MFGM) allow for an improved and more highly regulated immune response to LPS challenge. Grant Funding Source : National Dairy Council Project #1120 administered by Dairy Research Institute