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Effects of fat sources on high fat induced changes in inflammation and lipid metabolism in juvenile pigs (822.3)
Author(s) -
Jang Saebyeol,
Molokin Aleksey,
Urban Jeseph,
SolanoAguilar Gloria
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.822.3
Subject(s) - lipid metabolism , endocrinology , medicine , inflammation , adipose tissue , adipocyte , fat pad , biology , chemistry
Effects of fat sources on high fat induced changes in inflammation and lipid metabolism in juvenile pigs Saebyeol Jang 1 , Aleksey Molokin 1 , Joseph F. Urban, Jr. 1 , Gloria Solano‐Aguilar 1 : 1 USDA, ARS, Beltsville Human Nutrition Research Center, Diet, Genomics, and Immunology Laboratory, Beltsville, MD 20705 The goal of this study was to examine effect of anhydrous milk fat (AMF) and palm oil (PO) on the high fat induced changes in inflammation and lipid metabolism in juvenile pigs. Four‐week‐old conventional crossbred pigs were fed with either a low 10.8% fat (LF) or high 39.5% fat (HF) diets including AMF or PO as their main fat source for 18 weeks . Data was analyzed using Two‐Way ANOVA to determine main effect of fat source (AMF vs PO) or fat level (low vs high fat). Serum lipid panel analysis showed that cholesterol, HDL, and LDL levels were increased in high fat groups. In liver, expression of inflammatory genes including TNFα, iNOS and TLR4, was increased by high fat diets and mRNA expression of MCP‐1 was decreased in LF‐AMF and HF‐AMF (main effect of fat source, p = 0.01). High fat diets also decreased expression of lipogenic genes SCD1 and FASN in liver whereas increased SCD expression in isolated peripheral adipocyte. The mRNA expression for IL‐1β, TNFα, iNOS, COX2, TLR4, IL‐6, MCP‐1, ICAM and CD40 were increased in the stromal cell fraction associated with visceral fat from pigs consuming high fat diets. No significant main effect of fat source was detected in visceral or peripheral fat stromal fraction. Interestingly, hippocampal gene expression of TLR4, TNFα and MCP‐1 and serum endotoxin was lower in AMF groups showing main effect of fat source. Overall, this study showed that diet containing AMF attenuated inflammatory parameters in serum, liver and, brain compared with diet of PO in juvenile pigs. Grant Funding Source : Supported by DRI #1101