Premium
Tissue‐engineered extracellular matrices for 3D tissue modeling and clinical applications (82.2)
Author(s) -
Germain Lucie
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.82.2
Subject(s) - tissue engineering , extracellular matrix , dermis , regenerative medicine , stem cell , biomedical engineering , in vivo , in vitro , decellularization , ex vivo , regeneration (biology) , wound healing , acellular dermis , microbiology and biotechnology , chemistry , medicine , anatomy , biology , surgery , biochemistry , implant
Extracellular matrices (ECMs) are a key component of the tissue structure and influence cell responses and organ functionality. At the LOEX centre from Laval University, the self‐assembly approach for tissue engineering was designed to elaborate complex living substitutes based on the ability of cells to produce and organize their own extracellular matrices. Various tissues have been produced such as skin, cornea, blood vessels, etc without exogenous ECM or scaffolds. These natural ECMs favor the preservation of stem cells, facilitate vascularization and innervation, and provides adequate mechanical properties to these living three‐dimensional constructs. Moreover, this technique allows the fabrication of autologous substitutes. Tissue‐engineered skin, comprising a dermis and an epidermis, provides a permanent autologous graft for severely burned patients. No significant contraction was observed in vivo after grafting these tissue‐engineered skin substitutes. The presence of the ECMs promoted a good healing and suppleness after grafting. Thus, natural ECMs can be produced in vitro to generate tissue‐engineered substitutes for in vitro studies and clinical applications. Supported by the Canadian Institutes for Health Research (CIHR), Fonds de Recherche du Québec en Santé (FRQS), and the Cell and Tissue Therapy Network of the FRQS. LG is holder of the Chair on Stem Cells and Tissue Engineering.