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Effect of heat stress on expression of amino acid and glucose transporters in pigs (816.4)
Author(s) -
Cota Margarita,
Arce Néstor,
Castillo Gilberto,
Araiza Benedicto,
Cervantes Miguel,
Morales Adriana
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.816.4
Subject(s) - glut4 , duodenum , jejunum , glucose transporter , amino acid , transporter , heat stress , ileum , medicine , endocrinology , zoology , small intestine , amino acid transporter , chemistry , biology , biochemistry , insulin , gene
Animals exposed to high ambient temperatures (AT) suffer from heat stress (HS), which provokes physiological and metabolic changes. HS seems to affect the gastro intestinal tract integrity hence affecting absorption capacity. An experiment was conducted with 18 pigs (32.4±2.7 kg BW) to examine the effect of natural severe HS (AT up to 45 °C) on expression of amino acid (b0,+, CAT1) and glucose (SLC2A1, GLUT4) transporters in duodenum, jejunum, liver, and Longisimus (LDM) and Semitendinosus (STM) muscles. Treatments (T) were: T1, comfort pigs housed in AT controlled room (22±2 °C) and fed ad libitum (C‐AL); T2, pigs housed as in T1, but feed‐restricted (C‐FR); T3, pigs housed in a room with no climate control (AT raised up to 45 °C; HS). HS pigs consumed about 95% of their ad libitum feed intake, and feed intake of C‐FR was similar to that of HS pigs. All pigs received the same diet and purified water. HS increased relative expression of CAT‐1 (P=0.043) in liver, but reduced it in LDM (P=0.007). HS increased duodenal expression of SLC2A1 (P=0.041), and that of GLUT4 (P=0.063) in STM. Severe HS affects the expression of glucose and amino acid transporters in selected pig tissues. Grant Funding Source : Supported by CONACYT‐México