Premium
Chickens from lines selected for low or high body weight differ in fatty acid oxidation efficiency and metabolic flexibility in skeletal muscle and abdominal fat (814.2)
Author(s) -
Zhang Shuai,
McMillan Ryan,
Hulver Matthew,
Zhang Wei,
Siegel Paul,
Cline Mark,
Gilbert Elizabeth
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.814.2
Subject(s) - fatty acid , palmitic acid , skeletal muscle , endocrinology , medicine , chemistry , biology , biochemistry
The Virginia lines of chickens resulted from long‐term selection for low (LWS) or high (HWS) juvenile body weight. We hypothesized that hyperphagia and obesity in HWS are associated with reduced fatty acid oxidation efficiency and metabolic inflexibility in skeletal muscle and fat. Pectoralis major and abdominal fat were collected from 56‐day old LWS and HWS chickens (n=8). Production of 14CO2 and 14C‐labeled acid‐soluble metabolites (ASM) from the oxidation of [1‐14C] palmitic acid were measured. The ratio of CO2/ASM was indicative of oxidative efficiency. Fatty acid oxidation and metabolic flexibility were then measured in Pectoralis major and gastrocnemius skeletal muscles from 61 day‐old LWS and HWS chickens (n=7). Metabolic flexibility was determined from the decrease in pyruvate oxidation in the presence of free fatty acids. The rate of CO2 production was greater in 56 day‐old LWS than in HWS chickens in fat (P = 0.001), while the rate of ASM production was greater (P = 0.003) in the fat of HWS than LWS. The ratio of CO2/ASM production was greater (P < 0.0001) in the fat of LWS than HWS. Metabolic flexibility was greater (P = 0.006) in the Pectoralis major of LWS than in HWS. The results suggest that HWS chickens have impaired metabolic flexibility and oxidative efficiency in skeletal muscle and abdominal fat in comparison to LWS chickens.