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Anti‐adipogenic properties of selected phenolics in 3T3‐L1 cell model (811.31)
Author(s) -
Tan Huiyuan,
Tse Iris M.Y.,
Li Edmund T.S.,
Wang Mingfu
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.811.31
Subject(s) - adipogenesis , cell cycle , microbiology and biotechnology , chemistry , cell growth , 3t3 l1 , perilipin , cell , apoptosis , cyclin , lactate dehydrogenase , lipolysis , adipose tissue , biology , biochemistry , enzyme
Many phenolics are known to possess anti‐obesity properties through mechanisms that inhibit adipogenesis, stimulate lipolysis or induce apoptosis. This study aimed to examine the anti‐adipogenic effects of two phenolics, oxyresveratrol and cyanomaclurin in 3T3‐L1 preadipocytes. At doses that did not induce cytotoxicity (based on the lactate dehydrogenase assay), the exposure of oxyresveratrol (0‐100μM) or cyanomaclurin (0‐600μM) in the first three days of differentiation led to a dose‐dependent decrease in triglyceride accumulation on day 9 post‐confluent. Results of cell proliferation [3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐Diphenyltetrazolium Bromide (MTT) assay] and cell cycle [Fluorescence‐activated cell sorting (FACS) analysis] studies demonstrated that both compounds inhibited differentiation through inducing cell cycle arrest by retaining the preadipocytes in the G1 phase during the first two days post‐confluent. By studying the gene expression of the major transcriptional factors (Peroxisome proliferator‐activated receptor γ, CCAAT/ Enhancer‐binding proteins α), as well as their effects on related proteins expression in cell cycle modulation (cyclins and cyclin dependent kinase inhibitors), the regulatory pathways are identified. This research helps to identify novel agents with anti‐obesity properties by provide preliminary data on their mechanism of actions.