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Expression of microRNAs associated with apoptosis in neurospheres and adhered cells in glioblastoma cell line culture treated with ionizing radiation and temozolomida (803.14)
Author(s) -
Rodrigues Andressa,
Trevisan Felipe,
Peria Fernanda,
Carlotti Junior Carlos,
Tirapelli Daniela
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.803.14
Subject(s) - neurosphere , microrna , ionizing radiation , temozolomide , cell culture , cancer research , apoptosis , viability assay , cell , cell growth , radiosensitivity , radioresistance , biology , glioblastoma , medicine , radiation therapy , cellular differentiation , irradiation , genetics , gene , physics , adult stem cell , nuclear physics
Objectives: Evaluating the effect of ionizing radiation and temozolomide, alone or associated, on the expression of the miRNAs miR‐15, miR‐16 and miR‐21 in neurospheres and adhered cells in glioblastoma cell line (U343‐MG). Methods: The trypan blue was used to examine cell viability before and after treatment, and real time PCR to analyze the expression of microRNAs in time 0h (immediately after treatments), and 48h after the exposure to treatments. Results: The analysis of cell viability showed no statistically significant difference among the studied groups. The oncogene miR‐21 demonstrated a significantly higher expression in neurospheres in group treated with ionizing radiation (both 0h and 48h). The tumor suppressors microRNAs miR‐15 and miR‐16 had no significant expression among cell types and treatments modalities submissions. However, miR‐15 and miR‐16 had a considerably different expression between the analyzed periods: miR‐15 in the time 0h was highly expressed in adherent cells treated with ionizing radiation and temozolomide; and, during the 48 hour analysis, miR‐15 was more expressed in neurospheres with the same type of treatment. The same expression profile happened with the miR‐16, however being more expressed in the group treated with ionizing radiation. Conclusions: MicroRNAs studied in glioblastoma cell line culture demonstrated differentially expressions when compared neurospheres and adhered cells and different treatments. MiR‐21 expression was significantly different between cell types (neurospheres and adhered cells), remaining more expressed in neurospheres treated with ionizing radiation.