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Epigenetic regulation of EGF‐induced CTEN gene expression (800.2)
Author(s) -
Liao Yichun,
Chang Chiachun
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.800.2
Subject(s) - chromatin immunoprecipitation , epidermal growth factor , histone acetyltransferase , microbiology and biotechnology , biology , signal transduction , regulation of gene expression , epigenetics , cancer research , gene expression , cell culture , promoter , gene , genetics
CTEN induced by epidermal growth factor (EGF) mediates the EGF‐promoted cell migration and also prolongs EGF receptor (EGFR) signaling. However, the molecular mechanism underlying EGFR signaling upregulates CTEN expression is still incompletely understood. In this report, we showed that EGF‐induced expression of CTEN was mediated by ERK activation in a normal human prostatic epithelial cell line RWPE‐1 as well as in a cervical cancer cell line HeLa. Further dissection of the regulatory mechanism at transcriptional and posttranscriptional levels found that serial 5’‐deletions within 3‐kb CTEN promoter produced no significant induction in promoter activities after EGF stimulation. In addition, EGF has no effect on CTEN mRNA stability. Nonetheless, by chromatin immunoprecipitation assay, we revealed that EGF treatment enhanced the histone acetylation levels in the promoter region of CTEN . Furthermore, anacardic acid, a histone acetyltransferase p300 inhibitor, suppressed EGF‐induced CTEN expression, suggesting that the histone acetyltransferase p300 may be involved in the regulation of EGF‐induced CTEN expression. Grant Funding Source : Supported by National Science Council, the Republic of China (Taiwan)