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O‐GlcNAcylation modifies the metastatic properties of prostate cancer cells (789.5)
Author(s) -
Liu Xin,
Yang Pengyuan,
Hart Gerald
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.789.5
Subject(s) - lncap , prostate cancer , metastasis , cancer research , cancer , cancer cell , blot , biology , prostate , chemistry , biochemistry , genetics , gene
Prostate cancer is one of the most common forms of cancer in men and the second leading cause of cancer deaths in men in the United States. O‐linked β‐N‐acetylglucosamine (O‐GlcNAc) is a ubiquitous and dynamic post‐translational modification that exists on serine and threonine residues of nuclear and cytoplasmic proteins. This modification is regulated by O‐GlcNAc transferase (OGT), which attaches O‐GlcNAc to proteins, and O‐GlcNAcase (OGA), which removes O‐GlcNAc. O‐GlcNAc serves as a nutrient sensor to regulate virtually all cellular processes as well as playing roles in the various diseases, including Alzheimer's disease, diabetes, and cancer. The roles of O‐GlcNAc in the regulation of prostate cancer remains unclear. Herein, we investigate how the regulation of O‐GlcNAc may affect prostate cancer, progression using normal prostate epithelial cell (PrEc) and prostate cancer cell lines (LNCaP and PC‐3) as models. Using western blotting techniques, we show that O‐GlcNAc, OGT, and OGA are highly elevated in LNCaP and PC‐3 when compared to PrEc. LNCaP exhibited the highest levels of O‐GlcNAc, OGT, and OGA. OGT and OGA are differentially localized in lines with different metastatic properties. Additionally, 2D‐gel electrophoretic analyses show significant differences in O‐GlcNAcylated protein profiles among the three cell lines. In order to answer how O‐GlcNAc affects the biological properties of these cancer cells, including shapes, proliferation, invasion and metastasis, several assays were used. The result of soft agar assays indicate that alterations in O‐GlcNAc influence the metastasis ability of prostate cancer.