Premium
Sphingosine 1‐phosphate signaling regulates exosomal cargo sorting (783.3)
Author(s) -
Kajimoto Taketoshi,
Okada Taro,
Miya Satoshi,
Zhang Lifang,
Nakamura Shunichi
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.783.3
Subject(s) - endosome , microbiology and biotechnology , escrt , microvesicles , chemistry , förster resonance energy transfer , exosome , vesicle , receptor , biology , biochemistry , microrna , fluorescence , membrane , physics , quantum mechanics , gene
During late endosome maturation, cargo molecules are sorted into intralumenal vesicles (ILVs) of multivesicular endosomes (MVEs) and are either delivered to lysosomes for degradation or fused with the plasma membranes for exosome release. For delivering to lysosomes, the endosomal sorting complex required for transport (ESCRT) regulates both budding of ILVs and cargo sorting into ILVs. For exosome release, ceramide triggers budding of ILVs of MVEs, but the mechanism underlying cargo sorting into exosomal ILVs is still unclear. Here we show that inhibitory G protein (Gi)‐coupled sphingosine 1‐phosphate (S1P) receptors regulate exosomal cargo sorting. In concrete terms we demonstrated two major parts using fluorescence resonance energy transfer (FRET) technique, fluorescence recovery after photobleaching (FRAP) technique, or newly developed single exosome quantification technique; 1) Gi‐coupled S1P receptors on MVEs are constitutively activated through a constant supply of S1P on MVEs, 2) the continuous activation of Gi‐coupled S1P receptors on MVEs is essential for cargo sorting into ILVs destined for exosome release. Our results provide a new paradigm for a mechanism underlying cargo sorting into ILVs of exosomal MVEs.