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Biocompatible evaluation of fluorescent nanodiamonds in neurons in vitro (780.5)
Author(s) -
Kao JunWei,
Huang YungAn,
Chiang MingHan,
Huang HouSyun,
Liu KuangKai,
Chao JuiI,
Hwang Eric
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.780.5
Subject(s) - neurite , nanodiamond , nervous system , confocal microscopy , neuroscience , biology , chemistry , microbiology and biotechnology , in vitro , biochemistry , organic chemistry , diamond
In recent years, nanodiamond has emerged as a promising biomaterial for diagnostic and therapeutic applications. Fluorescent nanodiamond (FND) containing nitrogen‐vacancy centers is a new addition to the nanodiamond family. The uorophore produced by nitrogen‐vacancy made FND extremely photostable. Furthermore, the high refractive index makes FNDs visible under light microscopy. In previous studies, FND has been reported to have outstanding biocompatibility and high uptake rate in various cell types. However, the application of FND on neurons or nervous tissue remains elusive. In order to study the compatibility of FND on neurons, the viability and morphological alteration of neurons after treated with FND were examined. Dissociated primary neurons from both central nervous system (CNS) and peripheral nervous system (PNS) were cultured in the presence of FND and neither exhibited a reduction in viability. However, we did observe a FND dosage‐dependent decrease in neurite length in both CNS and PNS neurons. Time‐lapse live cell imaging suggested that the reduction of neurite length was due to the spatial hindrance of FND on advancing neuronal growth cone. Additionally, neuronal uptake of FNDs was confirmed using confocal microscopy and flow cytometry. These results indicated that FND exhibited low neuronal toxicity and can be readily taken into neurons. However, high dosage of FND may interfere with neurite elongation and should be taken into consideration when applications involve actively growing neurites (e.g. axon regeneration). Grant Funding Source : Supported by National Science Council of Taiwan