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Functionalizing silica‐coated iron oxide nanoparticles for imaging and targeted cancer therapeutics (780.3)
Author(s) -
Nagalla Raji,
Liao Zi Wei,
Webb Andrew,
Flynn Nolan,
Kolodny Nancy
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.780.3
Subject(s) - pancreatic cancer , drug delivery , nanotechnology , zeta potential , in vivo , polyethylene glycol , internalization , iron oxide nanoparticles , chemistry , cancer research , nanoparticle , surface modification , cancer , materials science , medicine , biochemistry , microbiology and biotechnology , cell , biology
This project focuses on the surface modification of silica‐coated superparamagnetic iron oxide NPs to achieve tissue specific drug delivery to pancreatic tumors. The treatment of pancreatic cancer remains a challenge in the biomedical community. Over half of cases are diagnosed after the cancer has spread to other tissues, and in these cases the 5‐year survival rate is 2%. The non‐specific nature of currently available chemotherapies has spurred interest in developing vehicles to deliver potent therapies specifically to tumors. Nanoparticles (NPs) present an ideal vehicle due to their high surface area, small size, and low toxicity. Recent work explored the conjugation of NPs to a targeting moiety: monoclonal antibody CHO 31.1, which recognizes gpA33, a membrane glycoprotein overexpressed in 50% of pancreatic cancers and 95% of colorectal cancers. Building on existing methods, the antibody was attached to the silica surface of the NPs via a polyethylene glycol (PEG) linker. In vitro techniques were also developed to quantify NP detection using magnetic resonance imaging (MRI). Attachment of multiple moieties was explored using orthogonal surface chemistries. Cellular internalization was determined using confocal microscopy. Moving forward these novel nanoparticles will be further characterized for morphology, size and zeta potential, and for their utility as contrast agents for tracking in vivo. Grant Funding Source : Supported by NIH grant #1R15CA156393‐01A1