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Evaluation of cytotoxicity of CdS/maltodextin nanoparticles bioconjugated with Cry protein in hepatic cells (780.1)
Author(s) -
Gutiérrez Ivonne,
ReyesEsparza Jorge,
RodriguezFragoso Patricia,
RodriguezFragoso Lourdes
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.780.1
Subject(s) - bioconjugation , cytotoxicity , chemistry , fluorescence microscope , fluorescence , biophysics , viability assay , cell , nanoparticle , nanotoxicology , microbiology and biotechnology , nanotechnology , biochemistry , toxicity , materials science , in vitro , biology , organic chemistry , physics , quantum mechanics
To evaluate of cytotoxicity of CdS/maltodextrin nanoparticles (CdS/MD) bioconjugated with Cry protein in hepatic cells. Previous studies have shown that CdS/MD nanoparticles are innocuous in several cell lines at low concentrations. For this study, the CdS/MD nanoparticles and bioconjugate were analyzed by fluorescence microscopy and electron microscopy. HepG2 cells were treated with CdS/MD alone and bioconjugated with Cry protein. We performed a dose‐response curve of CdS/MD and CdS/MD‐Cry protein [0.01, 0.1, 1‐9 μg/L] and cell viability was measured. The type of cell death was identified by fluorescence microscopy. It was found that the bioconjugate was 20 times bigger than the CdS/MD nanoparticles. It was shown that nanoparticles and the bioconjugate emit green fluorescence in cells HepG2. In cells treated with NPs was observed fully fluorescence, while those treated with the bioconjugate only was observed fluorescence in the cell membrane. The cytotoxicity assay showed that the nanoparticles and Cry protein were non‐toxic at low concentrations (<2μg/L). At low concentrations the bioconjugate did not produce toxicity in HepG2 cells (<0.1 μg/L). The Cry protein produced apoptosis while the bioconjugate produced necrosis. In conclusion, at low concentrations the bioconjugate did not produce toxicity in HepG2 cells. Grant Funding Source : CONACYT