Premium
Bioinformatics analysis of uman NOTCH4 protein (778.10)
Author(s) -
Bowman Jacob,
Tansey John,
Bennett Jennifer
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.778.10
Subject(s) - ankyrin repeat , notch signaling pathway , biology , computational biology , ankyrin , notch proteins , function (biology) , signal transduction , microbiology and biotechnology , genetics , bioinformatics , gene
NOTCH4 is a receptor protein in the highly conserved Notch signaling pathway that plays a critical role in many cellular processes, including differentiation and apoptosis. The structure of NOTCH1 has been determined and several functions elucidated. NOTCH1 is used as the model, in function and structure, for the rest of the Notch proteins. The objective of the current study is to use available bioinformatics data to better characterize functional domains and vital residues of the NOTCH4 protein. Amino acid sequence data of NOTCH4 were aligned against templates of homologous domains‐Ankyrin repeats, epidermal growth factor, and calcium‐binding epidermal growth factor ‐ from standards to find a predicted 3‐D structure of NOTCH4. The threading program RaptorX, has been used to generate a predicted structure of one domain of NOTCH4 and then Cn3D was used for visualization. This study plans to expand to identify important residues in all of the major domains. Data from this predicted structure will be used to inform future experiments and develop a clearer picture of the Notch signaling pathway.