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Time‐course analysis of gene expression in a mouse model simulating aspects of human post‐traumatic stress disorder (776.7)
Author(s) -
Muhie seid,
Srinivasan Seshamalini,
Gautam Aarti,
Chakraborty Nabarun,
Hammamieh Rasha,
Jett Marti
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.776.7
Subject(s) - prefrontal cortex , period (music) , transcriptome , neuroscience , circadian rhythm , biology , hippocampus , rhesus macaque , gene expression , glucocorticoid receptor , glucocorticoid , endocrinology , gene , immunology , genetics , physics , cognition , acoustics
We profiled transcriptome changes in brain regions and blood of socially defeated mouse model of post traumatic stress disorder. Brain regions involved in fear circuitry and blood were collected from 5 days and 10 days aggressor exposed and control mice. Changes in gene expression after 1 day, 10 days and 42 days of rest (post 5 days and 10 days defeat periods) were profiled using microarray and real time PCR. Differentially expressed blood transcripts were validated by assaying the corresponding plasma proteins. Functional and pathway analyses of differentially regulated genes in trauma response brain regions (Aygdala, hippocampus, medial prefrontal cortex, ventral striatum and nucleus acumbens) of defeated mice were found to be involved in startle response, associative learning, long term memory, inflammation, regulation of circadian rhythm, glucocorticoid receptor activity, dendritic branching, and synaptic depression. Blood transcripts with increased expression with longer rest period were involved in T‐cell immunity, serotonorgic and dopaminergic synaptic processes and axonal guidance. Transcripts in blood with decreased expression with longer rest period were involved in associative learning, opoid signaling and glutamatergic pathways. Changes at the transcriptome level indicated the pervasive nature of traumatic stress in altering functions and pathways which normally precipitate in phenotypic behavioral disorders and pathologies. With increased rest period, some of the important functions and pathways showed regaining vitality indicating a sense of recovery with time.