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Minotoring adenine nucleotides levels and their relevance to biochemical and cellular metabolic function using bioluminescence‐based strategies (766.5)
Author(s) -
Goueli Said,
Hsiao Kevin
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.766.5
Subject(s) - biochemistry , atpase , nucleotide , enzyme , adenine nucleotide , kinase , adenosine triphosphate , anabolism , atp hydrolysis , biology , microbiology and biotechnology , chemistry , gene
Adenine nucleotides are major determinants of the energy status of the cell and thus any modulation of their cellular concentration has significant consequences to cellular metabolism, cellular growth and cell death. ATP generating enzymes are usually involved in anabolic processes while ATP consuming enzymes are involved in catabolite processes. We have developed biochemical and cell based assays that monitor the concentrations of ATP, ADP and AMP in biochemical reactions and in cells. These universal assays can measure the activity of various enzymes with no modification of the native substrate and the ability to use diverse substrates such as proteins, peptides, lipids, sugars, etc. The AMP generating reactions such as ubiquitin ligases, aa aminoacyl t‐RNA synthetases, DNA ligases, cAMP‐dependent phosphodiesterases, etc. can be also monitored with high sensitivity and reproducibility. The ADP‐generating (kinases and ATPases, and ATP‐consuming reactions (kinases and ATPases) can be monitored as well using this luminescent approach. We will also show data using luminescent technology to monitor the cellular levels of these adenine nucleotides under various stress and physiological conditions.