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The effects of curcumin, ellagic acid and methionine on post‐thawed Merino rams sperm parameters (759.1)
Author(s) -
Omur Ali,
Coyan Kenan,
Ozturk Caner,
Gungor Sukru,
Bucak Mustafa
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.759.1
Subject(s) - ellagic acid , extender , sperm , curcumin , semen , methionine , chemistry , antioxidant , sperm motility , andrology , semen extender , food science , biochemistry , amino acid , polyphenol , medicine , organic chemistry , polyurethane
The aim of this study was to determine the effects of the antioxidants curcumin, ellagic acid and methionine on microscopic sperm parameters following the freeze‐thawing of Merino ram semen. Ejaculates were collected via artificial vagina from four Merino rams and microscopically evaluated and pooled at 37 ◦C. The pooled semen samples were diluted in a Tris‐based extender and separated to groups including curcumin (1, 2, 4 mM), ellagic acid (1, 2, 4 mM), methionine (1, 2, 4 mM) and no antioxidant (control). The diluted semen was slowly cooled to 5 ◦C and then the samples were frozen in liquid nitrogen vapour, plunged into liquid nitrogen (‐196ºC) for storage. Frozen straws were thawed individually at 37 ◦C for 30 s in a water bath for microscopic sperm evaluation. All of antioxidant groups led to higher percentage of sperm motility in comparison to the control group. The freezing extender supplemented with methionine (1 mM), curcumin (1 and 2 mM), ellagic acid (1 and 2 mM) led to higher percentage of sperm plasma membrane integrity when compared to the other groups (P < 0.05). Looking at the evaluation of sperm acrosome integrity, antioxidant groups led to higher percentace in comparison to the control. Methionine, curcumin and ellagic acid (1mM: 27.7±2.4, 28.0±2.1 and 26.8±2.0) groups provided more higher protective in terms of sperm mitochondrial activity when compared to the other groups (P < 0.05). Findings of this study showed that the different antioxidants (curcumin, methionine and ellagic acid) at different doses protective effects on the ram sperm parameters. Grant Funding Source : Selcuk University SCIENTIFIC RESEARCH PROJECTS COORDINATION UNIT (Project no: 09102041)